[BioChemistry] Academia Sinica's Biological Chemistry Researchers Find New Protein-DNA Interaction, Broaden Understanding of Molecular Mechanism of Antibiotic Resistance of Staph

[BioChemistry] Academia Sinica's Biological Chemistry Researchers Find New Protein-DNA Interaction, Broaden Understanding of Molecular Mechanism of Antibiotic Resistance of Staph (Chinese Version)

Academia Sinica Newsletter (2012/09/27) A recent study by a research team from the Institute of Biological Chemistry (IBC), Academia Sinica, led by Distinguished Research Fellow Andrew H.-J. WANG has increased understanding of the molecular mechanism of antibiotic resistance in the Staphylococci bacteria. The team found that multiple antibiotic resistance regulator TcaR in Staphylococcus epidermidis can bind to single-stranded DNA (ssDNA) and inhibit its replication. It is hoped that the findings, published online in the scholarly journal PLoS One on September 21, 2012, will aid the development of new treatments for Staphylococci infection.

The Staphylococci bacteria are one of the most common causes of bacterial infection. They can cause a wide variety of diseases in humans through invasion and toxin production, and also by producing biofilm to protect themselves from the host immune system and the action of antibiotics. Staphylococcus aureus is the most well-known species as it the cause of many antibiotic resistant hospital- and community-acquired infections. The protein TcaR from the multiple antibiotic resistance repressor (MarR) family of proteins is known to be responsible for the regulation of antibiotic resistance and biofilm formation in Staphylococci; however, the detailed mechanism of is action is unknown

In this study, the team used electrophoretic mobility shift assay (EMSA), circular dichroism (CD), and Biacore analyses to show that that the TcaR protein can interact strongly and cooperatively with ssDNA, thereby identifying a new role for MarR family proteins. In order to investigate the regulation mechanism of the ssDNA binding ability of TcaR, the team further used electron microscopy to reveal the TcaR-ssDNA complex. Their study also showed that TcaR could inhibit viral ssDNA replication and provide viral resistance against ssDNA phage in E. coli. Overall, the study suggests that TcaR plays of role in regulation of DNA replication.

The MarR family proteins are involved in multiple antibiotic resistance. They are sensors of changing environments, allowing pathogenic bacteria to survive and persist in a dynamic environment. Up to now, the knowledge of MarR family protein-nucleic acid interaction has been limited to double-strand DNA (dsDNA), this is the first study showing that an MarR family proteins also interact with ssDNA.

The research was conducted and financed by Academia Sinica and grants from the National Research Program for Biopharmaceuticals, a project funded by the National Science Council of Taiwan.

Related Website:
http://www.plos one.org.


Media Contacts:
Dr. Yu-Ming CHANG, Institute of Biological Chemistry, Academia Sinica
stanley039@yahoo.com.tw (Tel) +886-2-2785-5696 #5010
Ms. Mei-hui LIN, Office of the Director General, Central Office of Administration, Academia Sinica mhlin313@gate.sinica.edu.tw (Tel) +886-2-2789-8821 (Fax) +886-2-2782-1551 (M) 0921-845-234
Ms. Pearl HUANG, Office of the Director General, Central Office of Administration, Academia Sinica pearlhuang@gate.sinica.edu.tw (Tel) +886-2-2789-8820 (Fax) +886-2-2782-1551 (M)0912-831-188


Further Information:
Academia Sinica Newsletter 2012/09/27

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